restriction enzyme alwi  (New England Biolabs)

Journal: Genes

Article Title: PON1 , APOE and SDF-1 Gene Polymorphisms and Risk of Retinal Vein Occlusion: A Case-Control Study

doi: 10.3390/genes15060712

Figure Lengend Snippet: PCR-RFLP assays of paraoxonase 1 (PON1) Q192R after digestion of the amplified DNA by the AlwI restriction enzyme. Lanes 2, 4, 8, 9, 13—wild-type QQ genotype; Lanes 6, 7—mutant RR genotype; Lanes 1, 3, 5, 10, 11, 12, 14—heterozygous QR genotype. Lane L represents the φX174 (phiX174) Hae III digest DNA molecular weight marker ladder. The size of the restriction fragments is written with black letters on the left side of the figure.

Article Snippet: The NlaIII and AlwI restriction enzymes (New England BioLabs, Ipswich, MA, USA) were used to cleave the PCR products for the L55M and Q192R polymorphisms, respectively.

Techniques: Amplification, Mutagenesis, Molecular Weight, Marker

Journal: Health Science Reports

Article Title: CDKN2B‐AS (rs2891168), SOD2 (rs4880), and PON1 (rs662) polymorphisms and susceptibility to coronary artery disease and type 2 diabetes mellitus in Iranian patients: A case‐control study

doi: 10.1002/hsr2.1717

Figure Lengend Snippet: The primer sequences, melting temperature ( T m ), and product sizes for PCR‐RFLP and tetra ARMS‐PCR analysis.

Article Snippet: For rs662, the products were digested by AlwI restriction enzyme (Thermo Fisher Scientific) at 37°C for 24 h. The PCR product (for rs4880) and digested fragments (for rs662) were electrophoretically separated on 2.5% (w/v) agarose gel and then visualized by Safe Stain (Yekta Tajhiz Azma) under UV light using a gel documentation system (Bio‐Rad).

Techniques: Sequencing